Analysis of reverse-phase protein
Mathematical Systems Biology of Cancer
May 02,2006 09:40 AM to 10:10 AM
Speakers:
Baggerly, Keith
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Abstract: |
In recent years, high-throughput biological assays have been primarily focused on RNA
(microarrays). In this talk, we will describe how people are attempting to extend the high-throughput
paradigm to the exploration of the human proteome. Much of this talk is focused on trying to give a
clear explanation of how the various types of proteomic assays we have fit together, and how
measuring proteins is different from measuring DNA or RNA. To that end, I'm walking through the
basics of ELISAs, Western blots, and arrays, simply so that the combination in a reverse-phase
lysate array can be seen as an evolution of several processes. Having placed the assays in
context, we will look at some data generated at MD Anderson, and try to address a basic analysis
question: how do people quantify lysate array data? The hybridization kinetics on a reverse phase
array are more similar across an array than is the case with standard microarrays, so that
borrowing strength across samples within an array is more feasible. Borrowing across arrays, by
contrast, is less so. |
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