One common method to elucidate the presence and levels of proteins in cancer cell lines
is western blotting. Also known as immunoblots, they detect proteins in a given sample of tissue
with separation by gel electrophoresis and then probed by antibodies specific to the protein. This
talk will discuss the normalization of numerous sets of western blots on breast cancer cell lines
from the Gray lab. Each protein is detected across 38 breast cancer cell lines in a set of 4 blots.
To enable comparison across blots, a pair of cell lines are common in each blot. The samples are
adjusted on a multiplicative scale accordingly to the relationships between the common samples.

Lecture #12309

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